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目的： 用高效液相色谱法测定兔血清中琥乙红霉素的浓度。方法： 采用ＹＷＧ－ Ｃ１８ 色谱分析柱（４－６ ｍ ｍ×１５０ ｍ ｍ ，１０ μｍ）， 岛津ＳＰＤ－ ６ＡＶ 紫外检测器， 甲醇－ ０－０２ ｍｏｌ·Ｌ－ １ － 醋酸铵水溶液（７０∶３０ ， 用氨水调ｐＨ７－３） 为流动相， 红霉素作内标。血液样品在碱性条件下用二氯甲烷提取纯化后进样， 在２１０ ｎｍ 波长处检测。结果： 琥乙红霉素线性范围为０－２５～１２ ｍｇ·Ｌ－ １ （ｒ＝ ０－９９８ ９）， 最低检测量０－０２ μｇ， 最低检出浓度０－０５ ｍｇ·Ｌ－１ ， 平均方法回收率９６－２６％ 。精密度考察琥乙红霉素日内、日间相对标准偏差均小于６％ 。结论： 本方法快速、准确、重复性好。
Objective: To determine the concentration of erythromycin ethylsuccinate in rabbit serum by high performance liquid chromatography. Methods: The YWG-C18 column (4-6 m × 150 m m, 10 μm), Shimadzu SPD-6AV UV detector, methanol-0-02 mol·L -1 ammonium acetate aqueous solution 30, adjust pH7-3 with ammonia) as the mobile phase, erythromycin as internal standard. The blood samples were extracted with dichloromethane after purification under alkaline conditions and injected at 210 nm. Results: The linear range of erythromycin ethylsuccinate was 0-25 ~ 12 mg · L -1 (r = 0-998 9), the lowest detection limit was 0-02 μg, the lowest detection limit was 0-05 mg · L -1, The average recovery rate of 96-26%. The intra-day and inter-day relative standard deviations (RSDs) of erythromycin ethylsuccinate were all less than 6%. Conclusion: The method is rapid, accurate and reproducible.