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目的:观察环维黄杨星D(Cyclovirobuxine D,CVB-D)-甲醇结晶体溶液对大鼠单个心室肌细胞内向整流钾电流(in-ward rectifier current,IK1)的影响。方法:Langendorff灌流系统灌流大鼠离体心脏,急性酶解法获得单个心室肌细胞;全细胞膜片钳技术分别记录对照组、药物组及洗脱组的IK1曲线。结果:对于大鼠心肌细胞,在钳制电位-100 mV时,给予低浓度(4×10-4g/L)、高浓度(4×10-3g/L)CVB-D-甲醇结晶体溶液后,IK1内向电流峰值和内向电流稳态值与给药前相比均明显降低,IK1外向电流峰值也明显受到抑制。使用细胞外液灌流洗脱药液后,与给药后相比IK1内向电流峰值和内向电流稳态值均升高,IK1外向电流峰值升高显著。结论:4×10-4g/L组和4×10-3g/L组CVB-D-甲醇结晶体溶液对IK1内向电流成分和外向电流成分均具有可逆性抑制效应,这可能是其具有延长动作电位时间与抗心律失常作用的药理机制之一。
Objective: To observe the effect of Cyclovirobuxine D (CVB-D) -methanol crystalloid solution on in-ward rectifier current (IK1) in isolated rat ventricular myocytes. Methods: Langendorff perfusion system was used to perfuse the isolated rat heart. Single ventricular myocytes were obtained by enzymatic digestion. Whole cell patch clamp technique was used to record the IK1 curve of the control group, the drug group and the elution group, respectively. Results: For rat cardiomyocytes, IK1 (low concentration (4 × 10-4g / L) and high concentration (4 × 10-3g / L) Intrinsic current peak and inward current steady-state values were significantly lower than before administration, IK1 outward current peak was also significantly inhibited. When the extracellular fluid was used to elute the drug solution, the inward current peak value and the inward current steady state value of IK1 were all increased, and the peak value of IK1 outward current increased significantly. CONCLUSION: CVB-D-methanol crystalloid solution of 4 × 10-4g / L group and 4 × 10-3g / L group have a reversible inhibitory effect on IK1 inward current component and outward current component, which may be due to its prolonged action potential Time and anti-arrhythmic effect of one of the pharmacological mechanisms.