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Objective:To study the effects of simulated microgravity induced by tail-suspension on histological structures and antioxidant system in testis of adult rats.Methods:Forty Spraque-Dawley adult male rats were randomly divided into four groups,two experimental groups and two synchronic control groups.Rats in the two experimental groups were tail-suspended for 14 d and 28 d respectively,then serum hormone,testicular morphology and biochemical changes were evaluated.Results:Compared with the synchronic controls,serum testosterone(T)levels and testicular superoxide dismutase(SOD)significantly decreased(P < 0.05),while testicular malondialdehyde(MDA)significantly increased(P < 0.05)in tail-suspended rats.The changes were more prominent in the 28 d tail-suspended rats.In addition,hematoxylin and eosin(HE)staining showed that seminiferous tubules atrophied,spermatogenic cells decreased,and seminiferous epithelial cells disintegrated and shed,abnormal multinucleated giant cells appeared and the lumina were azoospermic in tail-suspended rats.Conclusion:Simulated microgravity induced by tail-suspension has a harmful effect on male reproduction of adult rats by decreasing the ability of testicular antioxidant defense.
Objective: To study the effects of simulated microgravity induced by tail-suspension on histological structures and antioxidant system in testis of adult rats. Methods: Forty Spraque-Dawley adult male rats were randomly divided into four groups, two experimental groups and two synchronic control groups . Results: Compared with the synchronic controls, serum testosterone (T) levels and testicular superoxide dismutase (R) in the two experimental groups were tail-suspended for 14 d and 28 d respectively, then serum hormone, testicular morphology and biochemical changes were obtained. (P <0.05) in tail-suspended rats. The changes were more prominent in the 28 d tail-born rats. In addition, hematoxylin and eosin (P < HE) staining showed that seminiferous tubules atrophied, spermatogenic cells decreased, and seminiferous epithelial cells disintegrated and shed, abnormal multinucleated giant cells appeared and the lumina were azoospermic in tail-suspended rats. Conflusion: Simulated microgravity induced by tail-suspension has a harmful effect on male reproduction of adult rats by decreasing the ability of testicular antioxidant defense.