丁磺氨酸增强氮芥和环磷酰胺对肿瘤细胞的杀伤作用(英文)

• 作 者: 张雪峰 姚明辉
• 机 构: 上海医科大学基础医学药理教研室!上海200032,上海医科大学基础医学药理教研室!上海200032
• 出 处: 中国临床药理学与治疗学杂志
• 发表日期: 1998年03期
• 关键词: 丁磺氨 杀伤作用 细胞毒作用 药物敏感性 生长抑制作用 白血病细胞 氮芥 谷胱甘肽 抑制率 细胞生长

目的研究谷胱甘肽合成抑制剂丁磺氨酸(BSO)对氮芥及环磷酰胺体内外细胞毒作用的影响。方法利用改良MTT法及瘤体称重法检测化疗药对肿瘤细胞生长的抑制作用。结果在体外BSO明显降低人HL_60白血病细胞中谷胱甘肽的含量 ,并显著增强氮芥的细胞毒作用 ,剂量修饰因子为9 19。在体内BSO能增强环磷酰胺对小鼠HepaA肝癌及Lewis肺癌的生长抑制作用 ,抑制率分别由26 1 %升至51 5 %,和由22 6 %升至32 3 %。结论BSO可增强氮芥和环磷酰胺对肿瘤细胞的体内外杀伤作用。

Objective To study the effects of glutathione (BSO), a synthetic inhibitor of glutathione, on the cytotoxicity of nitrogen mustard and cyclophosphamide in vitro and in vivo. Methods The inhibitory effect of chemotherapeutic drugs on the growth of tumor cells was detected by modified MTT method and tumor weighing method. Results In vitro, BSO significantly reduced the content of glutathione in human HL_60 leukemia cells and significantly enhanced the cytotoxicity of nitrogen mustard. The dose modification factor was 9 19. In vivo, BSO enhances the growth inhibitory effect of cyclophosphamide on HepaA and Lewis lung cancers in mice, and the inhibition rate increases from 261% to 515%, and from 226% to 32%. Conclusion BSO can enhance the killing effect of nitrogen mustard and cyclophosphamide on tumor cells in vitro and in vivo.