论文部分内容阅读
目的:研究siRNA干扰UGT1A1基因表达对HT-29细胞CPT-11化疗敏感性的影响。方法:realtime PCR,western blot方法检测UGT1A1 mRNA和蛋白表达。采用四甲基偶氮唑蓝显色法(MTT)观察HT-29细胞对CPT-11化疗敏感性的影响。结果:UGT1A1-siRNA可显著抑制HT-29细胞中UGT1A1 mRNA和蛋白的表达(P<0.01)。Con组与MOCK组IC50值分别为25.13和24.87,干扰组IC50值为12.23(P<0.05,VSCon或MOCK组)。结论:转染UGT1A1-siRNA可显着增加结直肠癌HT-29细胞对CPT-11化疗的敏感性。
AIM: To investigate the effect of UGT1A1 gene silencing on the sensitivity of HT-29 cells to CPT-11 chemotherapy. Methods: The mRNA and protein expression of UGT1A1 were detected by realtime PCR and western blot. The effect of HT-29 cells on chemosensitivity to CPT-11 was observed by MTT assay. Results: UGT1A1-siRNA significantly inhibited UGT1A1 mRNA and protein expression in HT-29 cells (P <0.01). The IC50 values of Con group and MOCK group were 25.13 and 24.87, respectively, and the IC50 value of interference group was 12.23 (P <0.05, VSCon or MOCK group). CONCLUSION: Transfection of UGT1A1-siRNA can significantly increase the sensitivity of colorectal cancer HT-29 cells to CPT-11 chemotherapy.