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目的 了解p16(MTS1)基因缺失在小儿急性淋巴细胞白血病(ALL)发病中的意义。方法 应用PCR和Southern 印迹杂交技术检测了47 例小儿ALL样品中p16基因的缺失。结果 全部被检样品中有9例存在p16 基因缺失,缺失率为19.1 % 。其中,8例T细胞型ALL(T-ALL)中有5 例显示缺失,缺失率为62.5 % ;39 例前B细胞型ALL(pre-B-ALL)中有4 例显示缺失,缺失率为10.3 % 。结论 p16 基因缺失与小儿ALL,特别是与T-ALL的发生或发展可能有密切关系
Objective To investigate the significance of deletion of p16 (MTS1) gene in the pathogenesis of pediatric acute lymphoblastic leukemia (ALL). Methods The deletion of p16 gene in 47 pediatric ALL samples was detected by PCR and Southern blot hybridization. Results There were 9 cases of p16 gene deletion in all the samples, the deletion rate was 19.1%. Among them, 5 of 8 T-ALL-type ALL (T-ALL) showed deletion, the deletion rate was 62.5%; 4 of 39 pre-B-ALL patients showed deletion and the rate of deletion was 10.3%. Conclusion The deletion of p16 gene may be closely related to the occurrence and development of T-ALL in children, especially the T-ALL