In vitro and in vivo trypanocidal action of aescin and aescin liposomes against Trypanosoma evansi i

• 作 者: Matheus Dellamea Baldissera Nathieli Bianchin Bottari Thirssa Helena Grando Roberto Christ Vianna Santos Ana Julia Figueiro Dalcin Patrfcia Gomes Renata Platcheck Raffin Carine Eloise Prestes Zimmerma
• 机 构: Department of Microbiology and Parasitology,Universidade Federal de Santa Maria(UFSM),Laboratory of
• 出 处: Asian Pacific Journal of Tropical Biomedicine
• 发表日期: 2014年12期
• 关键词: liposomes curative verify eliminating saponins parasite nanotechnology doses sto

Objective:To verify the trypanocidal effectiveness of aescin and aescin liposomes against Trypanosoma evansi in vitro and in vivo.Methods:Aescin and aescin liposomes were used in vitro on trypomastigotes at different concentrations(0.5%,1.0%and 2.0%) and exposure times(0,1,3,6 and 9 h).In vivo tests were performed using mice as the experimental model.Trypanosome evansi infected mice were treated with aescin and aescin liposomes with doses of 60 and 100 mg/kg during 4 d.Results:The three concentrations tested in free form and nanoencapsulated showed trypanocidal activity in vitro,completely eliminating the parasites in small concentration after6 h of assay.Animals treated with aescin(100 mg/kg) and aescin liposomes(100 mg/kg)showed increase in longevity,however without curative effect.Conclusions:Active compounds present in natural products,such as aescin,may potentiate the treatment of trypanosomosis when used in association with other trypanocidal drugs.

Objective: To verify the trypanocidal effectiveness of aescin and aescin liposomes against Trypanosoma evansi in vitro and in vivo. Methods: Aescin and aescin liposomes were used in vitro on trypomastigotes at different concentrations (0.5%, 1.0% and 2.0%) and exposure times ( 0, 1, 3, 6 and 9 h). In vivo tests were performed using mice as the experimental model. Trypanosome evansi infected mice were treated with aescin and aescin liposomes with doses of 60 and 100 mg / kg during 4 d. Results: The three concentrations tested in free form and nanoencapsulated showed trypanocidal activity in vitro, completely eliminating the parasites in small concentration after 6 h of assay. Animal sized treated with aescin (100 mg / kg) and aescin liposomes (100 mg / kg) showed increase in longevity , but without curative effect .Conclusions: Active compounds present in natural products, such as aescin, may potentiate the treatment of trypanosomosis when used in association with other trypanocidal drugs.