Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcin

• 作 者: Maram Shalabi Kh.Khilo Mahmoud M.Zakaria Mahmoud G.Elsebaei Walied Abdo Walaa Awadin
• 机 构: Department of Biochemistry, Faculty of Veterinary Medicine, Mansoura University,Department of Bioche
• 出 处: Asian Pacific Journal of Tropical Biomedicine
• 发表日期: 2015年05期
• 关键词: extracts Aloe apparently assessed sections stained eosin anticancer cytotoxic an

Objective: To investigate the in vitro anticancer effect of Aloe vera(A. vera) and Calligonum comosum(C. comosum) extracts against hepatocellular carcinoma(HepG 2) cells. Methods: Hep G2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG 2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic(Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG 2 cells were observed using light microscopy.Results: The IC50 values of A. vera and C. comosum extracts were(10.45 ± 0.31) and(9.60 ± 0.01) μg/mL respectively. The extracts separately increased cytotoxicity against HepG 2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions.Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.

Objective: To investigate the in vitro anticancer effect of Aloe vera (A. vera) and Calligonum comosum (C. comosum) extracts against hepatocellular carcinoma (HepG 2) cells. Methods: Hep G2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG 2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic (Bcl-2) were tested by real time PCR and flow cytometer analyzer. Hematoxylin and eosin stained sections from untreated and treated HepG 2 cells were observed using light microscopy. Results: The IC50 values ​​for A. vera and C. comosum extracts were (10.45 ± 0.31) and (9.60 ± 0.01) μg / mL respectively. The Extracts increased cytotoxicity against HepG 2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decreased Bcl-2 genes expressions. Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.