Highly Sensitive and Specific Monoclonal Antibody-Based Serological Methods for Rice Ragged Stunt Vi

• 作 者: LIU Huan SONG Xi-jiao NI Yue-q
• 机 构: InstituteofBiotechnology
• 出 处: 农业科学学报：英文版
• 发表日期: 2014年9期
• 关键词: 水稻褐飞虱 单克隆抗体 病毒检测 ACP-ELISA 血清学方法 异性 敏感性 IC-RT-PCR Rice ragged stunt virus rice b
• 基金项目: supported by the National Basic Research Program of China（2010CB126203）, the special fund for Agro-scientific Research in the Public Interest,China（201003031）, Earmarked Funds for Modern Agro-industry

Rice ragged stunt virus(RRSV) is a serious rice disease in Asia, causing serious yield losses on rice. The capsid protein(CP) gene of the major outer capsid protein of RRSV was expressed in Escherichia coli BL21(DE3) using the pMAL-C2 X expression vector.