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目的观察降压通络方对自发性高血压肾损害大鼠肾脏血管紧张素II(angiotensin II,Ang II)及肾功能的影响,探讨该方对高血压肾损害大鼠肾脏的保护机制。方法采用16周龄自发性高血压大鼠(Spontaneously Hypertension Rat,SHR)为研究对象,将其随机分为模型组、缬沙坦组、降压通络方高、中、低剂量组、并设正常血压大鼠(Wistar Kyoto,WKY)为空白对照,分别灌胃给药。于给药后4周和8周分别测定大鼠尾动脉压力、血肌酐、尿素氮及肾脏Ang II蛋白含量,并进行大鼠肾脏病理组织学检查。结果给药4、8周后,与模型组比较,各治疗组大鼠血压、肾脏Ang II含量明显降低(P<0.01,P<0.05),但给药4周各组之间比较无统计学意义,给药8周缬沙坦及降压通络方高、中剂量组均明显低于低剂量组(P<0.05);缬沙坦及降压通络方能显著降低模型组大鼠血肌酐、尿素氮(P<0.01,P<0.05),改善肾小动脉及肾小球硬化,促进肾组织结构的恢复。结论降压通络方能降低高血压肾损伤大鼠肾脏Ang II含量,从而有效降低大鼠血压,改善肾脏病理损害,保护肾功能。
Objective To observe the effects of “Antihypertension Tongluo Recipe” on renal angiotensin II (Ang II) and renal function in spontaneously hypertensive rats with renal damage and to explore its protective mechanism on the kidney of hypertensive renal injured rats. Methods Spontaneously hypertensive rats (SHR), 16 weeks old, were randomly divided into model group, valsartan group, high, medium and low dose of JSTT, Normal rats (Wistar Kyoto, WKY) as a blank control, were administered orally. The tail arterial pressure, serum creatinine, urea nitrogen and renal Ang II protein levels in rats were measured at 4 and 8 weeks after the administration, and pathological examination of rat kidneys was performed. Results At 4 and 8 weeks after administration, compared with the model group, blood pressure and renal Ang II levels were significantly decreased in all treatment groups (P <0.01, P <0.05), but there was no statistical difference between the four groups Significance, valsartan and antihypertensive Tongluo Recipe 8 weeks after administration were significantly lower than the low-dose group (P <0.05); valsartan and JSTT can significantly reduce the model group rats blood Creatinine, urea nitrogen (P <0.01, P <0.05), improve renal arterioles and glomerular sclerosis, and promote the recovery of renal tissue structure. Conclusion Xiaoyaadongluo can reduce renal hypertensive renal injury Ang II levels, thereby effectively reducing blood pressure in rats to improve renal pathological damage and protect renal function.