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目的 :探讨切断双侧颈部迷走神经对静注内毒素 (主要成分脂多糖Lipopolysaccharide,LPS)引起的大鼠肺部急性炎性损伤的影响。方法 :采用静注LPS(10mg/kg)引起大鼠急性肺脏炎性损伤的模型 ,分别切断双侧颈部迷走神经干或做假手术对照 ,在 1.5h检测各组动物血乙酰胆碱 (ACh)、皮质醇水平以及肺组织中肿瘤坏死因子 -α(TNF -α)、丙二醛 (MDA)量和髓过氧化物酶 (MPO)活性的变化。结果 :静注LPS1.5h即可引起肺部急性炎性病理改变 ,肺组织TNF -α、MDA量和MPO活性明显升高 (P <0 .0 1) ,血ACh和皮质醇也升高 (P<0 .0 5 ,P <0 .0 1)。静注LPS复合迷走神经切断组动物较单纯静注LPS动物肺组织中TNF -α、MDA量和MPO活性显著升高 (P <0 .0 5 ) ,肺组织炎性改变加重 ,而血ACh和皮质醇水平却显著下降 (P <0 .0 1)。结论 :迷走神经切断能加重肺组织的炎症反应和病理损害 ,迷走神经的完整性对于维持体内抗炎激素的水平 ,保护肺脏免受过度炎症的损害具有重要意义。
Objective: To investigate the effects of severing bilateral vagus nerve on acute lung injury in rats induced by intravenous injection of lipopolysaccharide (LPS). Methods: Acute lung injury induced by LPS (10mg / kg) was induced in rats. The bilateral cervical vagus nerve trunk was harvested or sham operated, respectively. The ACh, cortex Alcohol level and the changes of tumor necrosis factor-α (TNF-α), malondialdehyde (MDA) and myeloperoxidase (MPO) activity in lung tissue. Results: The acute inflammatory pathological changes of lungs were induced by intravenous injection of LPS for 1.5 hours, the levels of TNF-α, MDA and MPO in lung tissue were significantly increased (P <0.01), and the levels of ACh and cortisol in blood also increased P <0. 05, P <0. 01). The levels of TNF-α, MDA and MPO in the LPS combined with vagotomy group were significantly higher than those in the LPS alone group (P <0.05), and the inflammatory changes in the lung tissue were more severe than those in the LPS group Alcohol levels decreased significantly (P <0.01). Conclusion: Vagus nerve transection can aggravate inflammatory reaction and pathological damage in lung tissue. The integrity of vagus nerve is important for maintaining the level of anti-inflammatory hormone in the body and protecting the lung from the damage of excessive inflammation.