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棉酚是棉花重要的次生代谢产物之一,具有抗虫及抗病害的作用。法尼基焦磷酸合酶是杜松烯合酶的上游酶,催化合成法尼基焦磷酸,该物质是棉酚等次生代谢物生物合成的前体。本文依据亚洲棉法尼基焦磷酸合酶基因序列,采用同源RT-PCR技术,克隆了陆地棉法尼基焦磷酸合酶基因,对其序列和编码产物结构进行了分析。荧光定量PCR分析表明,该基因在棉花植株叶片表达量最高,根部最低,在花、铃、蕾、茎中的表达水平接近。以上工作为棉花异戊二烯基途径的分析及相关次生代谢的途径工程研究奠定了一定的理论基础。
Gossypol is one of the important secondary metabolites of cotton, which has the functions of insect resistance and disease resistance. Farnesyl pyrophosphate synthase, an upstream enzyme of the enzyme, catalyzes the synthesis of farnesyl pyrophosphate, a precursor of biosynthesis of secondary metabolites such as gossypol. Based on the sequence of fastigial pyrophosphate synthase gene from cotton, homologous RT-PCR was used to clone the farnesyl pyrophosphate synthase gene of G. hirsutum. The sequence and structure of the encoded product were analyzed. Fluorescent quantitative PCR analysis showed that the expression level of the gene in cotton leaves was the highest, lowest in roots, and close in flowers, bell, buds and stems. The above work has laid a theoretical foundation for the analysis of cotton prenyl-based pathway and related secondary metabolism pathway engineering.