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在体内,滋养层细胞是通过何种方式到达子宫螺旋动脉血管并对其基础结构进行重构的机制目前尚不清楚,但这一过程涉及到了细胞在血管内黏附和迁移的行为。采用微管吸吮技术和平行平板流动腔系统对整合素β1在滋养层细胞黏附和迁移中的作用进行检测。将滋养层细胞分别培养在I型鼠尾胶原和血管内皮细胞上,进行细胞黏附力测量和剪切应力加载,实验分为整合素β1抗体阻断组、非特异性抗体阻断组和正常对照组。结果显示:整合素β1抗体阻断组可显著降低细胞的黏附力和抵抗流体剪切应力引起位移的能力,而非特异性抗体组的结果与对照组差异不显著;内皮细胞可显著增强滋养层细胞的黏附力和抵抗流体剪切应力引起位移的能力。结果表明,整合素β1参与了滋养层细胞与血管内皮细胞间的黏附并调控了流体剪切应力作用下滋养层细胞的迁移行为。
In vivo, the mechanism by which trophoblast cells reach the uterine spiral arteries and reconstruct their basic structures is unclear, but this process involves the behavior of cells in the blood vessels to attach and migrate. The effect of integrin β1 on the adhesion and migration of trophoblast cells was detected by microtubule sucking technique and parallel plate flow cavity system. Trophoblast cells were cultured on type I collagen and vascular endothelial cells respectively, and cell adhesion assay and shear stress loading were carried out. The experiment was divided into blocking group of integrin β1 antibody, non-specific blocking group and normal control group . The results showed that integrin β1 antibody blocking group could significantly reduce cell adhesion and resistance to fluid shear stress induced displacement, while non-specific antibody group results and the control group was not significant; endothelial cells can significantly enhance the trophoblast cells Of adhesion and ability to resist displacement caused by fluid shear stress. The results show that integrin β1 is involved in the adhesion between trophoblast cells and vascular endothelial cells and regulates the migration of trophoblast cells under the action of fluid shear stress.