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目的观察异体角膜体外对人外周血Ｔ细胞及其亚群ＣＤ２８分子表达的调节。方法应用异体角膜与外周血淋巴细胞体外共同培养模型和流式细胞分析技术（ｆｌｕｏｒｅｓｃｅｉｎａｃｔｉｖａｔｅｄｃｅｌｓｏｒｔｅｒ，ＦＡＣＳ）。结果对照组Ｔ细胞ＣＤ２８表达为２１．３％；受角膜或沸波醇酯（ｐｈｏｒｂｏｌ１２，１３—ｄｉｂｕｔｙｒａｔｅ，ＰＤＢ）激活后，Ｔ细胞ＣＤ２８表达分别为５２．４％和８１．４％；受角膜激活后，ＣＤ４和ＣＤ８Ｔ淋巴细胞ＣＤ２８表达分别为６３．１％和５２．３％。结论角膜组织体外能够刺激人外周血Ｔ细胞ＣＤ２８表达和Ｔ细胞活化，ＣＤ４Ｔ细胞比ＣＤ８Ｔ细胞活化更明显。
Objective To observe the regulation of the expression of CD28 on T cells and their subpopulations in human peripheral blood in vitro. Methods The in vitro co-culture model of allogeneic cornea and peripheral blood lymphocytes and the flow cytometry (FACS) were used. Results The CD28 expression of T cells in control group was 21.3%. After activated by cornea or phorbol 12,13-dibutyrate (PDB), CD28 expression of T cells was 52.4% and 81.4% After activation, the CD28 expression of CD4 and CD8 T lymphocytes were 63.1% and 52.3%, respectively. Conclusions Corneal tissue can stimulate CD28 expression and T cell activation of T lymphocytes in vitro, and CD4 T cells activate more than CD8 T cells.