论文部分内容阅读
目的探讨载脂蛋白E短肽COG1410对蛛网膜下腔出血(subarachnoid hemorrhage,SAH)后早期脑损伤(early brain injury,EBI)中脑水肿、小鼠运动功能和水通道蛋白4(aquaproins-4,AQP-4)的影响。方法 108只C57BL/6J小鼠分成假手术对照组(sham)、生理盐水组(SAH+生理盐水)、治疗组(SAH+COG1410),采用血管内穿刺法建立SAH模型。观察小鼠运动功能变化,用干湿质量法测定脑含水量的变化,免疫组化法测定表达AQP-4阳性细胞数,酶联免疫吸附法(ELISA)测定AQP-4蛋白含量及实时定量PCR测定AQP-4 m RNA的变化。结果 SAH后生理盐水组和治疗组神经功能评分均明显高于假手术对照组(P<0.05),且生理盐水组和治疗组之间差异有统计学意义(P<0.05)。SAH后各时间点生理盐水组脑含水量、AQP4蛋白含量和AQP4 m RNA表达的变化与假手术对照组对比显著升高(P<0.05)。生理盐水组和治疗组脑含水量、AQP4蛋白含量和AQP4 m RNA表达均在SAH后1 d明显增加,2 d达到高峰,之后逐渐下降,2组间差异均有统计学意义(P<0.05)。结论载脂蛋白E短肽COG1410可能通过抑制AQP-4的过表达缓解EBI中的脑水肿。
Objective To investigate the effect of COG1410, an apolipoprotein E short peptide, on brain edema, early motor injury (EBI) in brain following subarachnoid hemorrhage (SAH), motor function and aquaproins-4 AQP-4). Methods 108 C57BL / 6J mice were divided into sham group, saline group (SAH + saline) and treatment group (SAH + COG1410). SAH model was established by intravascular puncture. The changes of motor function in mice were observed. The change of brain water content was measured by wet and dry mass method. The number of AQP-4 positive cells was determined by immunohistochemical method. The AQP-4 protein content was determined by enzyme-linked immunosorbent assay (ELISA) Changes in AQP-4 m RNA were measured. Results After SAH, the scores of neurological function in NS group and treatment group were significantly higher than those in sham operation group (P <0.05), and the difference between NS group and treatment group was statistically significant (P <0.05). After SAH, the changes of brain water content, AQP4 protein content and AQP4 mRNA expression in the saline group were significantly higher than those in the sham operation group at each time point (P <0.05). The brain water content, AQP4 protein content and AQP4 m RNA expression in NS group and treatment group both increased significantly 1 d after SAH and peaked on 2 d, then decreased gradually, with significant difference between the two groups (P <0.05) . Conclusion Apolipoprotein E short peptide COG1410 may relieve cerebral edema in EBI by inhibiting the overexpression of AQP-4.