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目的:优选大孔树脂吸附分离苹果原花青素的工艺条件。方法:选用3种不同极性大孔吸附树脂D113、DM130和ADS-17对苹果提取液中原花青素的吸附性能进行研究,以pH、样品流速、乙醇浓度、乙醇流速为考察因素,以吸附率和解吸率为评价标准。结果:D113树脂分离效果较好,吸附率达到68.1%,吸附量为5.91 mg·g-1树脂。最佳工艺是pH为7的原花青素粗提液以0.8 ml·min-1的流速上柱吸附,再用体积分数40%的乙醇、以0.8 ml·min-1的流速进行解吸。结论:D113大孔极性树脂适用于吸附分离苹果原花青素。
Objective: To optimize the process of macroporous resin adsorption and separation of procyanidins from apple. Methods: Three kinds of macroporous macroporous resins D113, DM130 and ADS-17 with different polarity were used to study the adsorption of proanthocyanidins in apple juice extract. Taking pH, sample flow rate, ethanol concentration and ethanol flow rate as the investigation factors, Desorption rate for the evaluation criteria. Results: The separation of D113 resin was better with the adsorption rate of 68.1% and the adsorption capacity of 5.91 mg · g-1 resin. The optimum conditions were as follows: the crude proanthocyanidin solution with pH of 7 was adsorbed on the column at a flow rate of 0.8 ml · min-1 and then desorbed at a flow rate of 0.8 ml · min-1 with 40% ethanol. Conclusion: D113 macroporous polar resin is suitable for the adsorption and separation of apple proanthocyanidins.