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本文叙述了用反相离效液桕包谱披术分析人体血清中的熊去氧胆酸(简称 UDCA),它的差向异构体鹅去氧胆酸(简称 CDCA),以及它们与甘氨酸及牛磺酸的结合型胆酸。此法可用于肝胆病人的诊断和病因研究。色谱条件;色谱柱采用不锈钢柱3.9×150mm,装填十八烷基键合相,Lichrosorb RP18,5μm。流动相为甲醇-磷酸二氢钾水液(30mmol/L)-高氯酸(76:24:0.02)。紫外可变波长检测器,测定波长201nm,流速,1.2ml/min。
This paper describes the use of reversed phase elution fluid chromatography spectrum analysis of human serum ursodeoxycholic acid (UDCA), its epimeric chenodeoxycholic acid (CDCA), and their glycine And taurine-bound cholic acid. This method can be used for diagnosis and etiology of hepatobiliary patients. Chromatographic conditions; column stainless steel column 3.9 × 150mm, packed octadecyl bonded phase, Lichrosorb RP18, 5μm. Mobile phase methanol - potassium dihydrogen phosphate solution (30mmol / L) - perchloric acid (76: 24: 0.02). UV variable wavelength detector, measuring wavelength 201nm, flow rate, 1.2ml / min.