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因抗EBV IgA的出现似乎是该病毒引起上皮细胞疾病的前导,作者研究了IgA是否介导EBV对上皮细胞的感染。用血凝素亲和层析、蔗糖密度梯度离心方法从急性传染性单核细胞增多症患者体内分离到EBV gp340的单体及多聚体IgA(pIgA),分别与HT-29.74细胞[人结肠癌细胞系,约20%细胞表面有丰富的复合型分泌小体(SC)]孵育,用免疫荧光检测发现只有pIgA能与细胞结
Since the appearance of anti-EBV IgA appears to be a precursor to the virus that causes epithelial cell disease, the authors investigated whether IgA mediates the EBV infection of epithelial cells. EBV gp340 monomeric and multimeric IgA (pIgA) were isolated from patients with acute infectious mononucleosis by hemagglutinin affinity chromatography and sucrose density gradient centrifugation, respectively, and compared with HT-29.74 cells [human colon Cancer cell lines, about 20% of the cell surface rich in complex secreted bodies (SC)] incubated with pIgA immunofluorescence assay found that only with the cell junction