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我们对东北四个地区的卫氏并殖吸虫囊蚴测量时发现,辽宁宽甸的囊蚴明显大于黑龙江鸡西市、伊春市和吉林桦甸县的囊蚴,前者的平均大小约为400μm,后三者的平均大小为300～350μm。为探讨上述四地卫氏并殖吸虫后尾蚴在形态上的差异,对其进行了扫描电镜观察。 材料与方法:卫氏并殖吸虫的囊蚴分别来自上述四地。囊蚴以0.5％胆汁脱囊,所获后尾蚴以2％胰蛋白酶液孵育30分钟并经生理盐水清冼后,先后置2.5％戊二醛液和1％锇酸液中固定,各级乙醇脱
When we measured Paragonimus westermani in four areas of northeast China, we found that the larvae of the larvae in Kuandian of Liaoning Province were significantly larger than the metacermicidal larvae in Jixi, Yichun and Huadian counties of Heilongjiang. The average size of the former was about 400μm, The average size of the latter three is 300 ~ 350μm. In order to investigate the morphological differences of the cercariae of Paragonimus in four areas mentioned above, we observed them by scanning electron microscopy. Materials and methods: Paragonimus westerman’s metacercaria were from the above four places. Cysticercus with 0.5% bile extirpation, the resulting cercariae 2% trypsin solution incubated for 30 minutes and cleared by saline, then set 2.5% glutaraldehyde solution and 1% osmium tetroxide fixed, all levels of ethanol Off