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目的:探讨聚合酶链反应顺序特异性寡聚核苷酸探针(polymerasechainreaction-sequencespe-cificoligonucleotideprobes,PCR-SSOP)反向杂交法应用于人类白细胞抗原(humanleukocyteantigen,HLA)-DRB1配型的可行性。方法:随机选取25例已行聚合酶链反应顺序特异性引物(polymerasechainreac-tion-sequencespecificprimers,PCR-SSP)法HLA-DRB1配型的肾移植受者的血标本,采用PCR-SSOP反向杂交法再次配型,并与PCR-SSP法的结果比较。结果:两法的一致性达92%。2例不一致的患者经再次PCR-SSOP反向杂交法配型后,其中1例与PCR-SSP结果一致,PCR-SSOP反向杂交法的平均操作时间为4小时30分。结论:PCR-SSOP反向杂交法是一种能以中等分辨度进行等位基因分型的方法,操作较简单,结果解读客观,适用于临床肾移植配型。
OBJECTIVE: To investigate the feasibility of using PCR-SSOP reverse hybridization to detect human leukocyte antigen (HLA) -DRB1. Methods: Twenty-five blood samples of renal transplant recipients with HLA-DRB1 matched with polymerase chain reaction-sequence specific primers (PCR-SSP) were randomly selected. The PCR-SSOP reverse hybridization Matched again and compared with PCR-SSP results. Results: The two laws are 92% identical. Two cases of inconsistent patients were reconstructed by PCR-SSOP reverse hybridization method. One case was consistent with PCR-SSP, and the average operation time of PCR-SSOP reverse hybridization was 4 hours and 30 minutes. Conclusion: PCR-SSOP reverse hybridization is a method of allelic typing with moderate resolution. The method is relatively simple to operate and objective interpretation of the results for clinical kidney transplantation.