论文部分内容阅读
目的:建立HPLC-DAD法比较采自23个产地的鼠尾草属6种植物根中丹参酮Ⅰ、丹参酮ⅡA、二氢丹参酮Ⅰ和隐丹参酮的含量。方法:Phenomenex Luna C18色谱柱(250 mm×4.6 mm,5μm);0.2%甲酸溶液(A)-0.2%甲酸甲醇溶液(B)为流动相,梯度洗脱;流速1.0 m L/min;柱温30℃;丹参酮Ⅰ、丹参酮ⅡA、二氢丹参酮Ⅰ和隐丹参酮的检测波长依次为246、270、240和264 nm。结果:4种成分的分离度良好,标准曲线在线性范围内相关性良好,平均加样回收率为93.1%~98.7%,RSD为0.37%~0.68%。结论:在分析的鼠尾草属6种植物中,采自西藏的野生栗色鼠尾草根中的4种丹参酮类成分含量较高。4种丹参酮类成分在不同物种不同产地样品间的含量存在明显差异,说明种间遗传差异和不同的环境条件差异都是影响丹参酮类化学成分含量的主要因素。
OBJECTIVE: To establish a HPLC-DAD method to compare the contents of tanshinone Ⅰ, tanshinone Ⅱ A, dihydrotanshinone Ⅰ and cryptotanshinone in root of 6 species of sage from 23 producing areas. METHODS: Phenomenex Luna C18 column (250 mm × 4.6 mm, 5 μm) was used as mobile phase with 0.2% formic acid (A) and 0.2% methanolic formic acid (B) 30 ℃. The detection wavelength of tanshinone Ⅰ, tanshinone Ⅱ A, dihydrotanshinone Ⅰ and cryptotanshinone were 246, 270, 240 and 264 nm in turn. Results: The separation of the four components was good. The calibration curve showed a good linear correlation. The average recovery was 93.1% ~ 98.7% with RSD 0.37% ~ 0.68%. CONCLUSIONS: Among the analyzed six species of Salvia, the content of four tanshinones in wild maroon sage roots collected from Tibet is high. The contents of four kinds of tanshinones in different samples from different areas were significantly different, indicating that genetic differences between species and different environmental conditions are the main factors that affect the chemical composition of tanshinones.