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有人认为,如数个卵同时受精,人胚冷藏能增加不孕夫妇通过体外授精—胚胎移植(IVF-ET)而妊娠的机会。然而冷藏的存活率至今仍很低,大概因为低温保护剂影响冻融后胚胎的成活率,或因其(甘油)仅适用于体外培养中存活4～5天胚胎。作者选择1,2-丙二醇作为低温保护剂,对1～2天胎龄胚的冷冻获得了满意的结果,使IVF周期中受精卵均能耐受冻融过程。为了选择生命力强的胚胎,以便立即移植到宫腔中或为以后移植备用冷冻,需要了解预定冻融受精卵的发育潜能与新鲜胚胎移植所得结果的比较。为此,作者比较了120个IVF周期所得的胚胎经冻
Some people think that if several eggs are fertilized at the same time, human embryo cryopreservation can increase the chance of pregnancy by infertile couples through IVF-ET. However, the survival rate of cold preservation is still low, presumably because the cryoprotective agent affects the survival rate of frozen-thawed embryos or because (glycerol) is only suitable for embryos that survive for 4 to 5 days in vitro culture. The authors chose 1,2-propanediol as a cryoprotectant and obtained satisfactory results on freezing embryos of 1 to 2 days so that the fertilized eggs in the IVF cycle can withstand the freeze-thaw process. In order to select viable embryos for immediate transplants into the uterine cavity or for subsequent replacement of frozen spares, a comparison of the developmental potential of a predetermined frozen-thawed fertilized egg with the results of a fresh embryo transfer is needed. To do this, the authors compared the freezing of embryos obtained from 120 IVF cycles