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卵泡闭锁是细胞程序化死亡即凋亡引起的。有关卵泡和颗粒细胞凋亡的调控机制很复杂,包括多种激素和生长因子,且多数为存活因素,促卵泡成熟激素(FSH)则是最重要的一种。有人用荧光显微镜研究表明体外受精(IVF)治疗有效者其颗粒细胞凋亡小体发生率低。如用流式细胞技术检测颗粒黄体细胞,其准确性将大大高于荧光显微镜。因它能在短时间内检测大量颗粒黄体细胞(>100 000)。本文研究用流式细胞技术测定的行IVF期间凋亡颗粒黄体细胞数与患者对治疗反应间的关系,以阐明凋亡细胞检测的
Follicular atresia is programmed cell death, apoptosis. The regulatory mechanisms involved in follicular and granulosa cell apoptosis are complex and include a variety of hormones and growth factors, mostly survival factors, and follicle stimulating hormone (FSH) is the most important. Fluorescent microscopy studies have shown that in vitro fertilization (IVF) treatment of effective rate of its granulosa apoptotic bodies is low. If using flow cytometry detection of granular luteal cells, its accuracy will be much higher than the fluorescence microscope. Because it can detect a large number of granular luteal cells in a short time (> 100 000). This article studies the relationship between the number of apoptotic particles and the response to treatment in IVF patients measured by flow cytometry to clarify the role of apoptotic cells in detecting