【摘 要】
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目的:探讨β-榄香烯对卵巢癌细胞SKOV3增殖和凋亡的影响。方法:体外培养卵巢癌SKOV3细胞,将β-榄香烯(浓度梯度为25,50,100,150,200μg/m L),单独作用于卵巢癌SKOV3细胞,加药
【机 构】
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陕西中医学院,解放军68222部队卫生队,第四军医大学唐都医院中医科暨中西医结合肿瘤科,第四军医大学西京医院中医科,第四军医大学军事预防医学系放射医学教研室,第四军医大学西京医院放疗科,
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目的:探讨β-榄香烯对卵巢癌细胞SKOV3增殖和凋亡的影响。方法:体外培养卵巢癌SKOV3细胞,将β-榄香烯(浓度梯度为25,50,100,150,200μg/m L),单独作用于卵巢癌SKOV3细胞,加药24 h、48 h后用噻唑蓝(MTT法)法检测细胞增殖情况;用流式细胞术检测加药24 h后对细胞凋亡的影响。结果:1MTT法结果显示β-榄香烯单独用药24 h、48 h后,与对照组相比,实验组对卵巢癌SKOV3细胞的抑制率均高于对照组(P<0.05),并且在一定程度上呈浓度和时间依赖性。2流式细胞术检测显示,β-榄香烯能够促进SKOV3细胞的凋亡。结论:β-榄香烯能够抑制卵巢癌细胞SKOV3的增殖,促进其凋亡。
Objective: To investigate the effects of β-elemene on the proliferation and apoptosis of ovarian cancer cell line SKOV3. Methods: SKOV3 cells were cultured in vitro. Β-Elemene (concentration gradient 25,50,100,150,200 μg / ml) was administered to SKOV3 ovarian cancer cells separately. After 24 h, the cells were treated with MTT assay ) Method was used to detect the proliferation of cells. Flow cytometry was used to detect the effect on apoptosis after 24 h. Results: The results of 1MTT assay showed that the inhibitory rate of experimental group on SKOV3 ovarian cancer cells was significantly higher than that of control group (P <0.05) at 24 h and 48 h after treatment with β-elemene alone, To a degree of concentration and time-dependent. 2 flow cytometry showed that, β-elemene can promote SKOV3 cell apoptosis. Conclusion: β-elemene can inhibit the proliferation and promote the apoptosis of ovarian cancer cell line SKOV3.
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