Concentration and Purification of Japanese Encephalitis Virus from Infected Mouse Brains

• 作 者: 马文煜 姜绍谆 于碧云 汪美先 李学荣 魏正乾
• 机 构: Department of Microbiology Fourth Military Medical College,Xi'an,Department of Microbiology,Fourth M
• 出 处: Journal of Medical Colleges of PLA
• 发表日期: 1986年04期
• 关键词: supernatant removed contaminated suspension concentrated Japanese clarified sucr

A suspension was prepared from the infant mouse brain in-fected by JEV.It was clarified by centrifugation.Protamine sulfatewas added to the supernatant with a final concentration of 5 mg/ml,and the contaminated protein was removed.The recovery of HAand infectivity was 66% and 55% respectively.The supernatant was concentrated by differential centrifugationand purified by rate zone centrifugation in sucrose density gradientwith recovery of 29% of HA and 37% infectivity.The purificationfactors(PF)were 138 for HA and 175 for infectivity.SHA without any infectivity were found on the top of gradientcolumn.The immunological characteristics of the componentsawait further studies.

A suspension was prepared from the infant mouse brain in-fected by JEV. It was clarified by centrifugation. Protamine sulfate was added to the supernatant with a final concentration of 5 mg / ml, and the contaminated protein was removed. The recovery of HA and infectivity was 66% and 55% respectively. The supernatant was concentrated by differential centrifugation and purified by rate zone centrifugation in sucrose density gradient with recovery of 29% of HA and 37% infectivity. Purificationfactors (PF) were 138 for HA and 175 for infectivity. any infectivity were found on the top of gradientcolumn. immunological characteristics of the componentsawait further studies.