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本文应用高压液相色谱法和紫外分光光度法测定犬和大鼠血浆及脑组织匀浆中的抗痫灵含量。 根据犬一次口服抗痫灵60mg/kg的血药浓度——时间消除曲线,按二房室开放模型,一级动力学推算出抗痫灵的药物动力学各项参数如下:峰浓度为8.8414μg/kg,峰时间为1.5小时,消除半衰期为9.2小时,消除速率为0.1096~(-h),血浆清除率为0.8329L/kg·h和表观分布容积为1.9426L/kg·h。抗痫灵片剂的相对生物利用度为93.94％。 大鼠一次口服抗痫灵400mg/kg的抗惊厥效应和血浆、脑组织中的药物浓度密切相关。抗痫灵的血浆和脑组织中浓度比值约为10(9.30～12.14),说明大鼠血浆中的浓度可以反映药物的抗惊厥药理效应。
In this paper, high-pressure liquid chromatography and UV spectrophotometry Determination of dogs and rats plasma and brain tissue homogenate anti-epileptic Ling content. According to the oral anti-epilepsy dog oral concentration of 60mg / kg-time elimination curve, according to two-bedroom open model, a kinetic deduced pharmacokinetics anti-epilepsy Ling parameters are as follows: peak concentration of 8.8414μg / kg, the peak time was 1.5 hours, the elimination half-life was 9.2 hours, the elimination rate was 0.1096 ~ (-h), the plasma clearance rate was 0.8329L / kg · h and the apparent volume of distribution was 1.9426L / kg · h. The relative bioavailability of anti-epilepsy tablets was 93.94%. The anticonvulsant effect of once oral anti-epilepsy 400mg / kg in rats was closely related to the drug concentration in plasma and brain tissue. The concentration ratio of anti-epilepsy in plasma and brain tissue was about 10 (9.30-12.14), indicating that the concentration in rat plasma can reflect the anticonvulsant pharmacological effect of the drug.