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目的:建立监测人血浆中咪达唑仑、三唑仑、艾司唑仑和阿普唑仑药物浓度的正相高效液相色谱方法。方法:血浆样品用乙醚萃取,55℃水浴中氮气吹干;残留物用乙醇溶解后进样。色谱柱为Shim-pack CLC-CN 柱(150 mm×6.0 mm,5μm),流动相为正己烷-无水乙醇-甲醇(76:18:6),流速为1.10 mL·min-1,柱温为:40℃,紫外检测波长为230 nm。结果:本方法可用于同时测定血浆中4种药物的浓度。咪达唑仑、三唑仑、艾司唑仑和阿普唑仑在0.050-20.0μg·mL-1范围内线性关系良好(r≥0.9996),准确度分别为98.9%,99.8%,101.8%及100.5%(n=6)。日内和日间RSD均不大于5.1%(n=6)。结论:本方法灵敏、特异性强、准确、简便易行,适用于咪达唑仑、三唑仑、艾司唑仑和阿普唑仑4种药物血浓度的临床监测。
Objective: To establish a method for the determination of midazolam, triazolam, estazolam and alprazolam in human plasma by normal phase high performance liquid chromatography. METHODS: Plasma samples were extracted with ether and dried in a nitrogen atmosphere at 55 ° C in a water bath. The residue was dissolved in ethanol and injected. The column was Shim-pack CLC-CN (150 mm × 6.0 mm, 5 μm). The mobile phase consisted of n-hexane-absolute ethanol-methanol (76:18:6) at a flow rate of 1.10 mL · min- : 40 ℃, UV detection wavelength of 230 nm. Results: The method can be used to simultaneously determine the concentration of four drugs in plasma. Midazolam, triazolam, estazolam and alprazolam had good linearity (r≥0.9996) in the range of 0.050-20.0μg · mL-1 with the accuracy of 98.9%, 99.8% and 101.8% And 100.5% (n = 6). RSDs are not greater than 5.1% for day and day (n = 6). Conclusion: The method is sensitive, specific, accurate, simple and easy to perform and is suitable for the clinical monitoring of blood concentration of 4 drugs including midazolam, triazolam, estazolam and alprazolam.