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目的探讨IFN-γ及TGF-β1、IL-10检测在儿童潜伏性结核感染诊断中的意义。方法采用ELISA法检测30例肺结核患儿(肺结核组)、30例潜伏性结核感染患儿及27例健康儿童(对照组)血清中IFN-γ、TGF-β1和IL-10水平。结果潜伏性结核感染组和结核组患儿血清IFN-γ分别为(38.17±10.73)pg/ml和(34.27±9.51)pg/ml,对照组为(48.33±14.51)pg/ml,差异有统计学意义(P<0.05);3组受检者血清TGF-β1、IL-10分别为(5.87±4.35)pg/ml、(13.12±5.40)pg/ml、(5.04±2.15)pg/ml和(49.06±17.89)pg/ml、(359.34±163.72)pg/ml、(46.18±14.53)pg/ml,差异有统计学意义(P<0.05)。结论血清IFN-γ测定有助于儿童潜伏性结核感染的诊断,但血清TGF-β1、IL-10水平对潜伏性结核感染的诊断意义不大。
Objective To investigate the significance of detection of IFN-γ, TGF-β1 and IL-10 in children with latent tuberculosis infection. Methods Serum levels of IFN-γ, TGF-β1 and IL-10 in 30 children with pulmonary tuberculosis (TB group), 30 children with latent tuberculosis infection and 27 healthy children (control group) were detected by ELISA. Results The serum levels of IFN-γ were (38.17 ± 10.73) pg / ml and (34.27 ± 9.51) pg / ml respectively in the latent tuberculosis and tuberculosis groups, and (48.33 ± 14.51) pg / ml in the control group (P <0.05). The serum levels of TGF-β1 and IL-10 in the three groups were (5.87 ± 4.35) pg / ml, (49.06 ± 17.89) pg / ml, (359.34 ± 163.72) pg / ml and (46.18 ± 14.53) pg / ml, respectively. There was significant difference between the two groups (P <0.05). Conclusions Serum IFN-γ is helpful for the diagnosis of latent tuberculosis infection in children. However, serum levels of TGF-β1 and IL-10 have little significance in the diagnosis of latent tuberculosis infection.