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目的:探讨雌马酚对大鼠脑基底动脉的舒张作用与机制。方法:应用微血管张力仪和全细胞膜片钳技术,分别观察无或有钾通道阻滞剂时,雌马酚对5-HT诱导的脑基底动脉收缩和血管平滑肌大电导钙激活钾通道(BKCa)电流的影响,并比较雌马酚对转染人BKCaα亚单位或α与β1亚单位共转染的HEK293细胞电流的作用。结果:雌马酚以非内皮依赖的方式,浓度依赖性(10-10mol/L~10-5mol/L)舒张大鼠脑基底动脉,其作用被BKCa阻断剂paxilline和iberiotoxin明显减弱,而不受电压依
Objective: To investigate the relaxation effect and mechanism of equol on cerebral basilar artery in rats. Methods: Microvascular tensiometer and whole-cell patch-clamp technique were used to observe the effect of equol on 5-HT-induced contractions of basilar artery and large-conductance calcium-activated potassium channel (BKCa) Currents and the effects of equol on the currents of HEK293 cells transfected with human BKCaα subunit or α and β1 subunits were compared. RESULTS: Equol deprived rat basilar artery in a concentration-dependent manner (10-10mol / L ~ 10-5mol / L) in a non-endothelium-dependent manner, and its effect was significantly attenuated by the BKCa blockers paxilline and iberiotoxin By voltage