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用改进的Ｋｌｅｎｋ法从狗红细胞中提取和纯化了神经节苷脂ＧＭ３，将分离纯化的ＧＭ３加入早幼粒白血病细胞系（ＨＬ－６０）ＤＭＥ／Ｆ１２培养液中，终浓度为５０μＭ，细胞的增殖明显受到抑制，并按单核－巨噬细胞分化成熟，表现为细胞吞噬功能显著增强，非特异性酯酶活性升高。细胞的形态呈现与分化相一致的变化，比较ＧＭ３诱导ＨＬ－６０细胞分化前后细胞中性糖脂图谱的变化，ＣＤＨ显著增多是ＧＭ３诱导ＨＬ－６０细胞向单核－巨噬细胞分化的一个重要特征，ＣＭＨ及ＣＱＨ亦相应显著增多。
The ganglioside GM3 was extracted and purified from dog erythrocytes using a modified Klenk method. The isolated and purified GM3 was added to the promyelocytic leukemia cell line (HL-60) in DME/F12 medium to a final concentration of 50 μM. Proliferation was significantly inhibited and mononuclear-macrophage differentiated and matured, showing a significant increase in phagocytosis and an increase in non-specific esterase activity. The morphology of cells showed changes consistent with differentiation. Compared to the changes in the neutrophil and lipid profiles of HL-60 cells before and after differentiation induced by GM3, the significant increase in CDH is an important factor in the differentiation of HL-60 cells into mononuclear macrophages induced by GM3. Characteristics, CMH and CQH also increased significantly.