论文部分内容阅读
目的对天津市2013年手足口病(HFMD)进行病原学检测,并对引起HFMD的病原体进行基因特征分析,为天津市HFMD的防控提供基础资料。方法采用real time RT-PCR方法对HFMD疑似病例标本进行核酸检测,对部分阳性标本进行病毒分离;并对病毒分离株进行分型鉴定和种系发生进化分析。结果 2013年共检测HFM D疑似病例1 728例,其中检出阳性1 390例,总阳性率为80.44%(EV71占16.38%,CVA16占11.46%,CVA6占43.92%,CVA10占1.56%,其他占7.12%);病毒分离株分型鉴定结果显示,获得的12个基因型别分别属于A、B两组肠道病毒;种系发生树分析显示在各基因型中,天津分离株与中国大陆或周边国家流行株亲缘关系较近。结论 2013年天津市HFMD流行的病原谱构成呈现多样性,CVA6是优势病原体。
Objective To analyze the etiology of hand, foot and mouth disease (HFMD) in 2013 in Tianjin and to analyze the genetic characteristics of the pathogens causing HFMD, so as to provide basic information for the prevention and control of HFMD in Tianjin. Methods The real-time RT-PCR method was used to detect the nucleic acid of suspected HFMD cases and to isolate some positive samples. The virus strains were identified and phylogenetically analyzed. Results A total of 1 728 suspected HFM D cases were detected in 2013, of which 1390 were positive. The total positive rate was 80.44% (16.38% in EV71, 11.46% in CVA16, 43.92% in CVA6 and 1.56% in CVA10) 7.12%). The typing results of virus isolates showed that the 12 genotypes belonged to group A and group B, respectively. The phylogenetic tree analysis showed that among all the genotypes, Epidemic strains of neighboring countries are more closely related. Conclusions The pathogenic spectrum of HFMD epidemic in Tianjin showed diversity in 2013, and CVA6 was the predominant pathogen.