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目的:探讨黄芪、灯盏花素复方制剂(HDs)对阿尔茨海默病(AD)大鼠红细胞、脑组织中超氧化物歧化酶(SOD)、过氧化脂质丙二醛(MDA)以及乳酸脱氢酶(LDH)的影响。方法:随机将50只大鼠分为正常对照组、脑复康阳性对照组(0.15 g·kg~(-1)·d~(-1),灌胃)、模型组以及HDs低剂量(HDs1,1.5 ml·kg~(-1)·d~(-1),腹腔注射)、HDs高剂量组(HDs 2,3.0 ml·kg~(-1)·d~(-1),腹腔注射),以三氯化铝(5 mg·kg~(-1)·d~(-1),灌胃)和D-半乳糖(40 mg·kg~(-1)·d~(-1),腹腔注射)建立AD大鼠动物模型。90 d后,测定各组大鼠的近期学习记忆能力,检测各组大鼠红细胞、脑组织SOD、MDA以及LDH水平。结果:与AD模型组比较,HDs各剂量组大鼠学习记忆能力明显提高,红细胞及脑组织中SOD、LDH的活性明显升高,MDA含量明显降低,差异均有统计学意义(P<0.05或P<0.01);但尚未恢复至正常水平(P<0.05或P<0.01)。HDs2组部分指标明显优于脑复康阳性对照药组和HDs1组(P<0.05或P<0.01)。结论:黄芪、灯盏花素复方制剂对AD大鼠的学习记忆障碍有明显的改善,能有效提高AD大鼠红细胞及脑组织SOD、及LDH的活性,降低MDA的浓度。
Objective: To investigate the effects of Astragalus and breviscapin (HDS) on the expression of superoxide dismutase (SOD), malondialdehyde (MDA) and lactate in erythrocyte and brain of Alzheimer disease (AD) Effect of Hydrogenase (LDH). Methods: Fifty rats were randomly divided into normal control group, Naofukong positive control group (0.15 g · kg -1 · d -1, intragastrically), model group and low dose HDs , 1.5 ml · kg -1 · d -1, intraperitoneal injection), HDs high dose group (HDs 2, 3 ml · kg -1 · d -1) , With aluminum trichloride (5 mg · kg -1 · d -1, gavage) and D-galactose (40 mg · kg -1 · d -1) The model of AD rats was established by intraperitoneal injection. After 90 days, the learning and memory abilities of rats in each group were determined, and the levels of SOD, MDA and LDH in erythrocytes and brain tissues were detected. Results: Compared with AD model group, the learning and memory abilities of HDs in each dose group were significantly increased, the activities of SOD and LDH in erythrocyte and brain tissue were significantly increased, while the content of MDA was significantly decreased (all P <0.05 or P <0.01), but not recovered to the normal level (P <0.05 or P <0.01). HDs2 group indicators were significantly better than the brain rehabilitation positive control group and HDs1 group (P <0.05 or P <0.01). Conclusion: Astragalus and breviscapin can significantly improve the learning and memory impairment of AD rats, increase the activity of SOD and LDH in erythrocytes and brain tissue, and decrease the concentration of MDA in AD rats.