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目的探讨芪丹颗粒剂预防性治疗矽肺纤维化的作用。方法 40只 Wistar 大鼠分为:正常对照组、模型组、芪丹组、汉甲组4个组。除正常对照组外,其余各组采用气管注射染尘(每只鼠50 mg)方法建立动物模型,芪丹组和汉甲组分别从造模后第2天灌注芪丹颗粒剂(3125 mg/kg)和汉防己甲素(22 mg/kg);5个月后处死,进行肺泡灌洗,取支气管肺泡灌洗液(bronchoalveolar lavagefluid,BALF)、肺脏、肾脏标本。称取大鼠体重、鼠肺湿重,计算大鼠肺系数;用碱水解法测定羟脯氨酸含量;用酶联免疫吸附测定法(ELISA)测定肺泡灌洗液中转化生长因子β1(TGF-β1)的表达;同时对肺组织作 HE 染色、VG 染色和网状纤维染色(gomorri),普通光镜下观察各组矽结节的细胞构成,胶原纤维及网状纤维改变;同时取肾组织作 HE 染色,普通光镜下观察肾脏病理改变。结果芪丹组、汉甲组的肺系数、羟脯氨酸含量、BALF 中 TGF-β1的表达均低于模型组,差异有统计学意义。模型组主要以Ⅲ～Ⅳ级矽结节为主,胶原纤维粗大、排列致密,网织纤维稀疏,芪丹颗粒及汉防已甲素预防治疗组主要以Ⅱ级矽结节为主,胶原纤维细小、排列疏松,网织纤维密集;汉甲组大鼠出现肾损伤,其余各组大鼠肾组织正常。结论芪丹颗粒剂有预防和延缓矽肺纤维化的作用,安全无肾毒性。
Objective To investigate the effect of Qidan granules in the prophylactic treatment of pulmonary fibrosis. Methods Forty Wistar rats were divided into four groups: normal control group, model group, Qidan group and Hanjia group. In addition to the normal control group, the other groups were treated with tracheal injection of dust (50 mg per rat) to establish an animal model, and the Dandan and Han Jia groups were infused with Qidan granules (3125 mg/day) from the second day after modeling. Kg) and tetrandrine (22 mg/kg); sacrificed 5 months later; bronchoalveolar lavage fluid (BALF), lung, and kidney specimens were taken for alveolar lavage. Weigh the weight of rats, the wet lung weight of rats, and calculate the lung coefficient of rats; Determine the content of hydroxyproline by alkaline hydrolysis; Determine the expression of transforming growth factor β1 in bronchoalveolar lavage fluid by enzyme-linked immunosorbent assay (ELISA). Expression of -β1); HE staining, VG staining, and reticular fiber staining (gomorri) were also performed on the lung tissue. The cell structure of the axillary nodules in each group was observed under ordinary light microscope. Collagen fibers and reticular fibers were changed; kidneys were also obtained. Tissues were stained with HE and pathological changes of the kidneys were observed under ordinary light microscope. Results The lung coefficient, hydroxyproline content and expression of TGF-β1 in BALF were lower in the Qidan group and the Hanjia group than in the model group. The difference was statistically significant. The model group mainly consisted of grade III-IV axillary nodules. The collagen fibers were thick and densely arranged, and the reticulated fibers were sparse. The Qidan Granules and the Hanban A-prevention treatment group mainly consisted of Class II axillary nodules. Collagen fibers Small, loosely arranged, densely reticulated fibers; renal damage occurred in rats of the Hanjia group, and normal renal tissues were observed in other groups. Conclusion Qidan granules have the effect of preventing and delaying fibrosis of the pneumoconiosis without any nephrotoxicity.