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为证实用日本血吸虫单克隆抗独特型抗体ＮＰ３０制备的抗体检测试剂盒考核治疗效果的价值，采用双“抗原”夹心酶联免疫吸附试验，用ＮＰ３０替代抗原，检测血吸虫病抗体。结果：该试剂盒对急性感染者的检出率为１００％，慢性轻度感染者的检出率为６１％，特异性为１００％。急性感染和慢性感染的Ｙｏｕｄｅｎ指数（ｒ）分别为１．０和０．６１，慢性感染者与经典抗体检测法ＳＥＡ／ＥＬＩＳＡ（０．６０）的ｒ无差别。用此试剂盒检测血吸虫病治疗后１ａ的血清，阴转率达１００％，明显优于经典抗体检测法（４３．６％）。结论：ＮＰ３０检测的抗血吸虫抗体属短程抗体，该试剂盒具有与循环抗原检测相同的考核疗效价值。
In order to verify the value of using the antibody detection kit prepared by the monoclonal anti-idiotypic antibody NP30 of Schistosoma japonicum to evaluate the therapeutic effect, double-antigen sandwich enzyme-linked immunosorbent assay was used to replace the antigen with NP30 to detect schistosomiasis antibodies. Results: The detection rate of the kit for acute infection was 100%. The detection rate of chronic mild infection was 61% and the specificity was 100%. The Youden index (r) for acute and chronic infections was 1.0 and 0.61, respectively, and no difference was found between chronic infection and r of the classical antibody test SEA / ELISA (0.60). Using this kit to detect serum 1a after schistosomiasis treatment, the negative conversion rate was 100%, which was significantly better than that of the classical antibody detection method (43.6%). CONCLUSIONS: The anti-schistosome antibodies detected by NP30 are short-range antibodies that have the same therapeutic value as circulating antigen assays.