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目的:探讨骨髓基质细胞诱导神经干细胞移植至KA大鼠海马后对学习记忆障碍的改善作用,从而为干细胞移植治疗癫痫提供理论依据。方法:首先分离幼年大鼠骨髓基质细胞,并在特定的条件下培养、诱导分化为神经干细胞。然后用KA建立大鼠癫痫模型,之后把神经干细胞移植至KA致痫鼠的海马内,用Morris水迷宫测试大鼠的学习记忆能力。结果:癫痫移植组与癫痫未移植组相比幼年大鼠空间学习记忆能力有显著性差异(P<0.05)。结论:骨髓基质细胞诱导神经干细胞移植至KA幼年大鼠海马后对学习记忆障碍有明显的治疗作用,但其具体的作用机理有待进一步研究。
OBJECTIVE: To investigate the effect of bone marrow stromal cells (MSCs) transplantation on hippocampus of hippocampus in KA rats on the improvement of learning and memory impairment, so as to provide a theoretical basis for stem cell transplantation in the treatment of epilepsy. Methods: Bone marrow stromal cells of juvenile rats were isolated and cultured under specific conditions to induce differentiation into neural stem cells. The model of epilepsy was established by KA. After that, the neural stem cells were transplanted into the hippocampus of KA-induced epileptic rats, and the learning and memory ability of rats was tested by Morris water maze. Results: Compared with epilepsy group, the spatial learning and memory ability of young rats in epilepsy group was significantly different (P <0.05). CONCLUSION: Bone marrow stromal cells can significantly reduce the learning and memory impairment after transplantation of neural stem cells into the hippocampus of KA juvenile rats. However, its specific mechanism remains to be further studied.