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目的:观察辛伐他汀对糖尿病肾病(diabetic nephropathy,DN)大鼠尿蛋白及尿足细胞排泄的影响。方法:雄性SD大鼠运用STZ法制作DN模型,设立正常对照组、DN组及辛伐他汀处理组。于实验第12周收集大鼠24h尿检查尿白蛋白,间接免疫荧光法检测大鼠尿沉渣的podocalyxin用于检测尿中足细胞的数量。结果:他汀药物组与糖尿病肾病模型组(DN组)相比,空腹血糖(FBG)无明显下降,但总胆固醇(TCHO)、血肌酐(Scr)、24h尿蛋白定量均显著下降。同时,对照组SD大鼠尿中很少见到足细胞的排泄,而DN组尿排泄足细胞显著增多,应用辛伐他汀治疗12周后,排泄足细胞数虽然仍比正常对照组高,但与DN组相比明显减少,差异有显著性。结论:糖尿病大鼠尿足细胞排泄明显增加,而辛伐他汀处理可减少尿蛋白及足细胞的排泄,可保护肾功能。
Objective: To observe the effect of simvastatin on urinary protein and urinary podocyte excretion in rats with diabetic nephropathy (DN). Methods: Male Sprague-Dawley rats were made DN model by STZ method and normal control group, DN group and simvastatin treatment group were established. Urine albumin was collected at 24h after urine test in rats and podocalyxin was detected by indirect immunofluorescence assay in urinary podocytes. Results: Compared with diabetic nephropathy model group (DN group), statin group had no significant decrease in fasting blood glucose (FBG), but the total cholesterol (TCHO), serum creatinine (Scr) and urinary protein in 24 hours decreased significantly. In the meantime, there was seldom excretion of podocyte in the urine of the control group SD rats, while the excretion of podocytes in the DN group was significantly increased. After 12 weeks of simvastatin treatment, although the number of excreted podocytes was still higher than that of the normal control group, Compared with the DN group was significantly reduced, the difference was significant. Conclusion: The excretion of urinary podocytes in diabetic rats increased significantly, while simvastatin treatment could reduce urinary protein and podocyte excretion and protect renal function.