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Reid等应用流式细胞计数对在组织中细胞的DNA含量加以测定,认为增殖性的增加及非整倍体可用以鉴定Barrett粘膜,后者是癌发展的一种高危险因素。食管的Barrett化生与发展成腺癌的危险性增加相关,不过总的发生率看来相当低。因此在估价Barrett食管方面寻找一个高危粘膜的可靠标志意义很大。Reid等称增殖增加或非整倍体,或两者与Barrett粘膜异型增生相关。作为诊断试验,非整倍体或G_2/M期细胞的增加,或两者均增加,与异型增生或癌的存在密切相关。活检中发现异倍体或G_2/M细胞群大于6%对于识别癌和异型增生有80%的敏感性及90%的特异性。需要注意的是异倍体对癌前(或早癌)粘膜的敏感性不见得比对确定为癌的更高。因为大多数癌仅有60%～80%为异
Reid et al. used flow cytometry to determine the DNA content of cells in tissues, suggesting an increase in proliferation and aneuploidy that could be used to identify Barrett’s mucosa, which is a high risk factor for cancer development. The Barrett’s metaplasia of the esophagus is associated with an increased risk of developing adenocarcinoma, but the overall incidence appears to be rather low. Therefore, it is of great significance to look for a reliable marker of high-risk mucosa in the evaluation of Barrett’s esophagus. Reid et al. said that increased proliferation or aneuploidy, or both, is associated with dysplasia of Barrett’s mucosa. As a diagnostic test, an increase in aneuploidy or G2/M phase cells, or both, is associated with the presence of dysplasia or cancer. An aneuploid or G2/M cell population greater than 6% was found on biopsy to be 80% sensitive and 90% specific for identifying cancer and dysplasia. It should be noted that the sensitivity of aneuploids to precancerous (or early cancer) mucosa is not higher than that determined to be cancerous. Because most cancers are only 60% to 80% different