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Objective:To observe the protective effects of safflor Injection(SI) and extract of Ginkgo biloba(EGB) on lung ischemia-reperfusion injury(LIRI) and investigate its mechanism.Methods:In vivo rabbit model of LIRI was reconstructed.Forty rabbits were randomly and equally divided into four groups:sham-operation group(sham group),ischemia-reperfusion group(model group),ischemia-reperfusion plus SI group(safflor group) and ischemia-reperfusion plus EGB injection group(EGB group).Malondialdehyde(MDA) content,superoxide dismutase(SOD) and xanthine oxidase(XO) activity in serum were measured.The wet/dry weight ratio(W/D) of the lung tissue and activity of myeloperoxidase(MPO) were also tested.Ultrastructure change of the lung tissue was observed by the electron microscope.The expression of intercellular adhesion molecule-1(ICAM-1) was measured by immunohistochemistry(IHC).Results:In the model group,MDA and XO increased and SOD decreased in serum compared with the sham group(P<0.01).The values of W/D,MPO and ICAM-1 of the model group were higher than those of the sham group(P<0.01),but those of the safflor group and EGB group were significantly lower than those of the model group(P<0.01).The IHC demonstrated that ICAM-1 expression in lung tissue of the model group was significantly higher than those of the safflor group(P<0.01).Compared with safflor group,in the EGB group MDA,XO,MPO decreased,SOD and ICAM-1expression increased(P<0.05),but the change of W/D was not statistically significant(P>0.05).Conclusions:SI and EGB may attenuate LIRI through antioxidation,inhibition of neutrophil aggregation and down-regulation of ICAM-1expression.But EGB had more effect on the antioxidation,while SI did better on regulating ICAM-1 expression.
Objective: To observe the protective effects of safflor injection (SI) and extract of Ginkgo biloba (EGB) on lung ischemia-reperfusion injury (LIRI) and investigate its mechanism. Methods: In vivo rabbit model of LIRI was reconstructed. Forty rabbits were randomly selected and equally divided into four groups: sham-operation group (sham group), ischemia-reperfusion group (model group), ischemia-reperfusion plus SI group (safflor group) and ischemia-reperfusion plus EGB injection group The content of superoxide dismutase (SOD) and xanthine oxidase (XO) activity in serum were measured. The wet / dry weight ratio (W / D) of the lung tissue and activity of myeloperoxidase (MPO) also also tested. Ultrastructure change of the lung tissue was observed by the electron microscope. The expression of intercellular adhesion molecule-1 (ICAM-1) was measured by immunohistochemistry (IHC). Results: In the model group, MDA and XO increased and SOD decreased in serum compared with the sham group (P <0.01). The values of W / D, MPO and ICAM-1 of the model group were higher than those of the sham group (P <0.01), but those of the safflor group and EGB group were significantly lower than those of the model group ). IHC demonstrated that ICAM-1 expression in lung tissue of the model group was significantly higher than those of the safflor group (P <0.01) .Compared with safflor group, in the EGB group MDA, XO, MPO decreased, SOD and ICC-1 expression increased (P <0.05), but the change of W / D was not significant significant (P> 0.05). Conclusions: SI and EGB may attenuate LIRI through antioxidation, inhibition of neutrophil aggregation and down-regulation of ICAM- 1 expression .But EGB had more effect on the antioxidation, while SI did better on regulating ICAM-1 expression.