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目的:对黔产药用植物泽漆Euphorbia helioscopia L.中的化学成分以及其体外抗肿瘤作用进行研究。方法:应用正、反相硅胶(RP-18型)柱层析;葡聚糖凝胶(Sephadex LH-20型)柱层析,结合重结晶方法进行分离纯化,运用1H-NMR、13C-NMR、ESI-MS和EI-MS等现代波谱技术对化合物的结构进行鉴定;采用磺酰罗丹明蛋白染色法对部分化学成分进行体外抗肿瘤活性的测试。结果:从黔产药用植物泽漆乙醇提取物中分离纯化了9个化合物,分别鉴定为:euphohelioscopin A(1)、euphoscopin(2)、9,19-cyclolanost-23E-ene-3,25-diol(3)、euphoscopin C(4)、euphornin A(5)、euphoheliosnoid A(6)、ent-kaurane-3-oxo-16β,17-diol(7)、9,19-cyclolanost-25-ene-3β,22-diol(8)和helioscopinolide A(9);化合物9对人乳腺癌细胞株MCF-7的增殖有一定的抑制作用。结论:其中,化合物3、7、8和9为首次从泽漆中分离得到,化合物9具有潜在的抗肿瘤活性。
OBJECTIVE: To study the chemical constituents of Euphorbia helioscopia L. and its antitumor activity in vitro in Guizhou medicinal plants. METHODS: The compounds were separated and purified by RP-18 column chromatography and Sephadex LH-20 column chromatography with the method of recrystallization. The structures of the compounds were characterized by 1H-NMR and 13C-NMR , ESI-MS and EI-MS and other modern spectroscopic techniques to identify the structure of the compounds; sulfonyl rhodamine protein staining of some of the chemical constituents in vitro anti-tumor activity test. Results: Nine compounds were isolated and purified from the ethyl alcohol extract of medicinal plant Qian in Guizhou Province and identified as euphohelioscopin A (1), euphoscopin (2), 9,19-cyclolanost-23E-ene-3,25- diol (3), euphoscopin C (4), euphornin A (5), euphoheliosnoid A (6), ent- kaurane- 3-oxo- 16β, 17- diol (7), 9,19-cyclolanost- 3β, 22-diol (8) and helioscopinolide A (9). Compound 9 can inhibit the proliferation of human breast cancer cell line MCF-7. Conclusion: Among them, compounds 3, 7, 8 and 9 were isolated from Zeman for the first time, and compound 9 possessed potential antitumor activity.