【摘 要】
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Vitellin (Vn) homeostasis is central to the fecundity of oviparous insects.Most studies have focused on the synthesis and transportation of Vn as a building block for developing eggs during vitellogenesis;however,less is known about how the utilization of
【机 构】
：
College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,China;Key Laboratory of M
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Vitellin (Vn) homeostasis is central to the fecundity of oviparous insects.Most studies have focused on the synthesis and transportation of Vn as a building block for developing eggs during vitellogenesis;however,less is known about how the utilization of this nutrient reserve affects embryonic development.Here,we show that the single or-tholog of the knirps and knirps-like nuclear receptors,KNRL,negatively regulates Vn breakdown by suppressing the expression of hydrolase genes in the brown planthopper,Nilaparvata lugens.KNRL was highly expressed in the ovary of adult females,and knock-down of KNRL by RNA interference resulted in the acceleration of Vn breakdown and the inhibition of embryonic development.Transcriptome sequencing analysis revealed that numerous hydrolase genes,including cathepsins and trypsins were up-regulated af-ter KNRL knockdown.At least eight of the nine significantly enriched Gene Ontology terms for the up-regulated genes were in proteolysis-related categories.The expression levels of five selected trypsin genes and the enzymatic activities of trypsin in the em-bryos were significantly increased after KNRL knockdown.Moreover,trypsin injection prolonged egg duration,delayed embryonic development,accelerated Vn breakdown and severely reduced egg hatchability,a pattem similar to that observed in KNRL-silenced N.lugens.These observations suggest that KNRL controls Vn breakdown in embryos via the transcriptional inhibition of hydrolases.Generally,this study provides a founda-tion for understanding how embryo nutrient reserves are mobilized during embryogen-esis and identifies several genes and pathways that may prove valuable targets for pest control.
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