【摘 要】
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Transcription factor Broad Complex (BR-C) is an ecdysone primary response gene in insects and participates in the regulation of insect growth and development.In this study,we performed a genome-wide identification of BR-C target genes in silkworm(Bombyx m
【机 构】
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State Key Laboratory of Silkworm Genome Biology,Biological Science Research Center,Southwest Univers
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Transcription factor Broad Complex (BR-C) is an ecdysone primary response gene in insects and participates in the regulation of insect growth and development.In this study,we performed a genome-wide identification of BR-C target genes in silkworm(Bombyx mori) using chromatin immunoprecipitation followed by high-throughput se-quencing (ChIP-seq).As a result,a total of 1006 BR-C ChIP peaks were identified,and 15％ of peaks were located in the promoter regions of 133 protein-coding genes.Func-tional annotation revealed that these ChIP peak-associated genes,as potential BR-C tar-gets,were enriched in pathways related to biosynthetic process,metabolic process,and development.Transcriptome analysis and quantitative real-time polymerase chain reac-tion (PCR) examination revealed that developmental changes in expression patterns of a portion of potential BR-C targets,including HR96 and GC-α1,were similar to those of BR-C.ChIP-PCR examination confirmed that BR-C could directly bind to the promot-ers of potential targets.Further,dual luciferase assays demonstrated that HR96 promoter activity was significantly upregulated following BR-C overexpression,and this upregu-lation was abolished when the binding motif in the promoter was truncated.This study will be helpful for deciphering the regulatory roles of BR-C during insect growth and development.
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