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在碱性介质中,发现依诺沙星对Luminol-H2O2化学发光体系有很强的增敏作用。基于此,提出了一种流动注射化学发光测定依诺沙星的新方法。对检测条件进行了优化。在优化条件下,依诺沙星的线性范围为1.5×10-8～2.0×10-7mol/L,检出限为2.8×10-9mol/L。对1.0×10-7mol/L依诺沙星平行测定11次,其RSD为1.7%。将此方法成功应用于注射用葡萄糖依诺沙星、依诺沙星滴眼液和依诺沙星片3种实际样品中依诺沙星含量的测定。结合化学发光光谱和紫外可见吸收光谱,对该反应机理进行了探讨。
In alkaline medium, Enoxacin was found to have a strong sensitizing effect on Luminol-H2O2 chemiluminescence system. Based on this, a new method for the determination of enoxacin by flow injection chemiluminescence was proposed. The test conditions were optimized. Under the optimized conditions, the linear range of enoxacin was 1.5 × 10-8 ~ 2.0 × 10-7mol / L, the detection limit was 2.8 × 10-9mol / L. The RSD of 1.0 × 10-7mol / L enoxacin was determined in parallel for 11 times with a RSD of 1.7%. The method was successfully applied to the determination of enoxacin in three kinds of real samples of injection of enoxacin, enoxacin and enoxacin. Combined with chemiluminescence and UV-Vis absorption spectra, the reaction mechanism was discussed.