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目的研究白细胞介素1B基因(IL-1B)启动子区域-31位点和-511位点及白细胞介素1受体拮抗剂基因(IL-1RN)多态性在我国北方人群胃癌患者与胃炎患者中的分布,探讨各基因型与胃癌的相关性。方法收集126例胃癌患者与125例慢性胃炎患者(对照组)的外周血标本和流行病学资料,提取基因组DNA；IL-1RN基因采用PCR方法直接测定,IL-IB-31基因采用PCR-CTPP方法,IL-1B-511基因采用PCR-RFLP的方法进行基因分型。通过快速尿素酶、~(14)C呼气试验及Hp血清IgG抗体的方法检测Hp感染。结果IL-IRN有5种基因型,分别为1/1、1/3、1/4、1/2和2/2型,其出现频率在胃癌组中分别为76．19%、4．76%、6．35%、11．90%和0．79%；在对照组分别为76．00%、4．00%、4．80%、13．60%和1．60%。各基因型在胃癌组和对照组中分布差异无统计学意义。IL-1B-31位点有3种基因型C/C、C/T和T/T型,在胃癌组中的频率分别为12．70%、47．62%和39．68%；在对照组中的频率分别为28．00%、48．80%和23．20%。与C/C型相比较,携带T/T基因型者胃癌发生的风险增加,OR=3．772(95%CI=1．786-7．966)。IL-1B-511位点有3种基因型C/C、C/T和T/T型,在胃癌组中的频率分别为19．20%、56．80%和24．00%；在对照组中的频率分别为23．38%、49．19%和27．42%。各基因型在胃癌组和对照组中分布差异无统计学意义。结论IL-1B基因启动子区域-31位点的基因多态性可能与国人胃癌易感性相关；尚未有证据表明IL-1RN和IL-1B-511位点的基因多态性与国人胃癌易感性相关。
Objective To investigate the polymorphisms of IL-1B locus and IL-11 locus and interleukin-1 receptor antagonist gene (IL-1RN) in patients with gastritis The distribution in patients, to explore the correlation between genotype and gastric cancer. Methods Genomic DNA was extracted from peripheral blood samples from 126 patients with gastric cancer and 125 patients with chronic gastritis (control group). IL-1RN gene was directly detected by PCR and IL-1B-31 gene was detected by PCR-CTPP Methods: The IL-1B-511 gene was genotyped by PCR-RFLP. Hp infection was detected by rapid urease, ~ (14) C breath test and Hp serum IgG antibody. Results The genotypes of IL-IRN were 1/1, 1/3, 1/4, 1/2 and 2/2 and their frequencies were 76.19% and 4.76 %, 6.35%, 11.90% and 0.79% in the control group and 76.00%, 4.00%, 4.80%, 13.60% and 1.60% respectively in the control group. There was no significant difference in the distribution of genotypes between gastric cancer group and control group. There were three genotype C / C, C / T and T / T genotypes in IL-1B-31 locus, and the frequencies in gastric cancer group were 12.70%, 47.62% and 39.68% The frequency in the group was 28.00%, 48.80% and 23.20%, respectively. Compared with C / C genotype, the risk of gastric cancer with T / T genotype increased, OR = 3.772 (95% CI = 1.786-7.966). There were three genotype C / C, C / T and T / T genotypes in the IL-1B-511 locus, with frequencies of 19.20%, 56.80% and 24.00% in the gastric cancer group respectively. The frequency in the group was 23.38%, 49.19% and 27.42%, respectively. There was no significant difference in the distribution of genotypes between gastric cancer group and control group. CONCLUSION: Gene polymorphism at locus-31 of IL-1B gene promoter may be related to gastric cancer susceptibility in Chinese people. There is no evidence that gene polymorphisms of IL-1RN and IL-1B-511 loci are associated with gastric cancer susceptibility in Chinese Related.