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目的探讨胃癌组织中血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)-C和生存素(survivin)蛋白表达及其临床意义。方法采用免疫组织化学SP法检测97例原发性胃癌组织、癌旁组织及20例正常胃黏膜组织中VEGF-C和survivin蛋白的表达,并分析其与临床病理特征和预后的关系。结果胃癌组织VEGF-C和survivin蛋白表达阳性率分别为66.0%和57.2%,显著高于癌旁组织和正常胃组织(P<0.05);VEGF-C蛋白表达与肿瘤分化程度、肿瘤部位、肿瘤直径、静脉侵犯及远处转移等无关,但与淋巴结转移、淋巴管侵犯、浆膜面受累和肿瘤TNM分期等密切相关;survivin蛋白表达与肿瘤分化程度、肿瘤部位、肿瘤直径、静脉侵犯等无关,但与浆膜面受累、淋巴管侵犯、淋巴结转移、远处转移和肿瘤TNM分期等密切相关;VEGF-C和survivin阳性表达组术后生存率明显低于阴性组;VEGF-C和survivin在胃癌组织中阳性表达呈正相关。结论VEGF-C及survivin蛋白的阳性表达可作为胃癌预后不良的参考指标。
Objective To investigate the expression of vascular endothelial growth factor (VEGF) -C and survivin in gastric carcinoma and its clinical significance. Methods The expressions of VEGF-C and survivin protein in 97 cases of primary gastric cancer tissues, adjacent non-cancerous tissues and 20 cases of normal gastric mucosa tissues were detected by immunohistochemical SP method. The relationship between the expression of VEGF-C and survivin protein was analyzed. Results The positive rates of VEGF-C and survivin protein expression in gastric cancer tissues were 66.0% and 57.2%, respectively, which were significantly higher than those in paracancer tissues and normal gastric tissues (P <0.05). The expression of VEGF-C was correlated with tumor differentiation, tumor location, Diameter, venous invasion and distant metastasis, but not related to lymph node metastasis, invasion of lymphatic vessels, serosal surface involvement and tumor TNM stage. Survivin expression was not associated with tumor differentiation, tumor location, tumor diameter, venous invasion , But closely related to serosal surface involvement, invasion of lymphatic vessels, lymph node metastasis, distant metastasis and tumor TNM stage. The survival rates of VEGF-C and survivin positive groups were significantly lower than those of negative ones. VEGF-C and survivin Positive expression of gastric cancer was positively correlated. Conclusion The positive expression of VEGF-C and survivin protein can be used as a reference index for poor prognosis of gastric cancer.