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以转IrrE基因与非转基因甘蓝型油菜为材料,用10%(W/V)PEG6000(聚乙二醇)进行了干旱胁迫实验,比较研究了转IrrE基因与非转基因甘蓝型油菜幼苗的耐受性应答情况。结果表明:转基因油菜植株含水量下降趋势小于非转基因油菜植株;转基因油菜植株叶绿素含量和荧光参数F0的上升趋势均明显大于非转基因油菜植株;转基因油菜植株的Fv/Fm值和可溶性蛋白含量均大于非转基因油菜植株,但丙二醛(MDA)含量小于非转基因油菜植株。以上结果从侧面证实了:在干旱胁迫下,转IrrE基因油菜植株较非转基因植株有更强的耐受能力;IrrE基因作为一种转录调节因子,广泛参与了油菜幼苗对干旱胁迫的耐受性应答过程,增强了植株的耐旱能力。
Irradiation with IrrE gene and non-transgenic Brassica napus was conducted under drought stress with 10% (W / V) PEG6000 (polyethylene glycol), and the tolerance of IrrE gene to non-transgenic Brassica napus seedlings was compared Sexual response situation. The results showed that the water content of transgenic plants decreased less than that of non-transgenic plants; the content of chlorophyll and the rising trend of fluorescence parameters F0 of transgenic plants were significantly higher than that of non-transgenic plants; the Fv / Fm and soluble protein contents of transgenic plants were greater than Non-transgenic rapeseed plants, but malondialdehyde (MDA) content is less than non-transgenic canola plants. The above results confirmed from the side: under drought stress, IrrE gene rape plants have stronger tolerance than non-transgenic plants; IrrE gene as a transcriptional regulator, widely involved in rapeseed seedling tolerance to drought stress The response process enhances the plant’s ability to tolerate drought.