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采用厌氧菌培养技术对不同阶段的３２名单纯母喂养儿和２４名混合喂养儿粪便的双歧杆菌和肠杆菌进行了分离、培养、计数和鉴定。结果显示：出生５～７天的母乳喂养儿粪便优势菌为双歧杆菌，肠杆菌数量远远低于双歧杆菌，而混合喂养儿粪便优势菌为肠杆菌，双歧杆菌数量明显少于肠杆菌。母乳喂养儿双歧杆菌数量主于混合喂养儿，肠杆菌则低于混合喂养儿。根据此结果，出生５～７天的母乳喂养儿肠道以无合成维生素Ｋ能力的双歧杆菌为优势菌，有维生素Ｋ２俣成量有限，又因母乳喂养，乳汁维生素Ｋ１提供不足，结果出现维生素Ｋ两个来源都匮乏的局面。混合喂养儿不仅外源性维生素Ｋ摄入量较多，而且优势菌肠杆菌内源性维生素Ｋ合成量亦丰富，因此，混合喂养儿维生素Ｋ来源充足
Anaerobic culture was used to separate, culture, count and identify 32 stage pure mother-fed infants and 24 mixed-breed bifidobacteria and enterobacteria in different stages. The results showed that the predominant bacteria in feces of breastfeeding children aged 5-7 days were Bifidobacterium, the number of Enterobacteriaceae was much lower than that of Bifidobacterium, while the dominant feces of mixed feeding feces was Enterobacteriaceae, the number of Bifidobacteria was significantly less than that of intestinal Bacillus. Breastfeeding Bifidobacterium is mainly the number of mixed feeding children, Enterobacteriaceae is lower than the mixed feeding children. Based on this result, bifidobacteria, which have no ability to synthesize vitamin K, are predominant bacteria in the intestine of breast-fed infants born 5-7 days after birth. There is a limited amount of vitamin K2, and due to breastfeeding and insufficient supply of milk vitamin K1, the result appears Vitamin K two sources are scarce situation. Mixed feeding is not only more exogenous vitamin K intake, and dominant bacteria Enterobacteriaceae endogenous vitamin K synthesis is also rich, therefore, mixed feeding children vitamin K sufficient sources