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目的了解致肾盂肾炎大肠杆菌(UPEC)毒力因子基因的分布。方法用PCR方法对41株UPEC菌株和40株健康人粪便分离的大肠杆菌进行5种毒力因子基因及新基因R049的扩增检测,并进行对比分析。结果 UPEC组和健康人粪便分离大肠杆菌6种毒力因子基因papC、fimH、hly、cnf1、aer和R049的检出率分别为100%和2.5%,53.66%和5%,63.41%和10%,34.15%和5%,60.98%和35%,40%和7.5%;前者高于后者(P<0.01)。结论 UPEC毒力因子基因分布呈现多态性。
Objective To understand the distribution of virulence factors genes of pyogenic pyogenic E. coli (UPEC). Methods Five strains of E.coli isolated from feces of 41 strains of UPEC and 40 healthy individuals were amplified by polymerase chain reaction (PCR) and their gene R049 was amplified and compared. Results The positive rates of papC, fimH, hly, cnf1, aer and R049 genes in E.coli isolated from feces of UPEC and healthy individuals were 100% and 2.5%, 53.66% and 5%, 63.41% and 10%, respectively , 34.15% and 5%, 60.98% and 35%, 40% and 7.5% respectively. The former was higher than the latter (P <0.01). Conclusion UPEC virulence factor gene distribution showed polymorphism.