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目的观察及评价甲苯胺红不加热血清试验(TRUST)、酶联免疫吸附试验(ELISA)、胶体金法与明胶颗粒凝集试验(TPPA)4种方法联合检测梅毒血清学试验的效果。方法对本院2011年-2013年进行梅毒项目检测的70 265例住院患者的血清进行初筛,确诊的1 682例不同期梅毒患者的血清以TRUST、ELISA、胶体金法与TPPA联合筛查检测,4种试验中2种及以上试验阳性,判别为联合筛查试验阳性;以TPPA检测作为对照,计算4种方法检测各期梅毒的阳性率,对联合筛查试验和对照试验组结果进行比较分析。结果 TRUST各期梅毒的阳性率均低于其他3种方法,差异有统计学意义(P<0.05);ELISA、胶体金法潜伏期阳性率低于TPPA,差异有统计学意义(P<0.05);各期梅毒联合筛查阳性率与TPPA法对照组比较,经χ2检验结果显示2种试验结果一致。结论 TRUST、ELISA、胶体金法与TPPA联合筛查各期梅毒可以取长补短,结合病史与临床症状,可以进一步监测病情和疗效预测。
Objective To observe and evaluate the effects of TRUST, ELISA, colloidal gold and gelatin particle agglutination test (TPPA) on syphilis serology. Methods The sera of 70 265 hospitalized patients who underwent the syphilis project from 2011 to 2013 in our hospital were screened. The serum levels of 1 682 syphilis patients diagnosed in different stages were detected by TRUST, ELISA, colloidal gold and TPPA , Four kinds of tests in two or more tests were positive, judged as a positive screening test; TPPA test as a control, calculated four kinds of methods to detect the positive rate of syphilis, the combined screening test and control group results were compared analysis. Results The positive rate of syphilis in each stage of TRUST was lower than that of the other three methods (P <0.05). The positive rate of ELISA and colloidal gold latency was lower than that of TPPA (P <0.05). The positive rate of syphilis screening in each phase was compared with TPPA control group. The results of χ2 test showed that the two tests were consistent. Conclusion TRUST, ELISA, colloidal gold and TPPA combined screening of syphilis can complement each other, combining history and clinical symptoms, further monitoring of disease and efficacy prediction.