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本文报道尖吻蝮蛇毒凝血酶样酶对犬及家兔的亚急性毒性及生化药理效应。用药量(μg/kg·d)犬分别为200、65,家兔分别为540、180,连续静脉注射28天。用药后14天及28天,WBC,DC,Hb 含量、肝、臂功能均无明显异差。血浆纤维蛋白原含量明显降低,凝血酶样酶时间及凝血酶时间明显延长,血小板聚集功能明显抑制,高剂量组个别犬血浆尿素氮显著上升(停药2天后降低),实验结束时体重均增加。用药后10天起,个别犬于每天注药后出现短时反应,至3周后消失。病理解剖检查眼肉及镜检未见重要脏器发生病变。本资料提示凝血酶样酶是一低毒高效应制剂,用药2周后对血小板聚集的抑制作用有耐受现象,因此连续用药时间不宜过长。
In this paper, subacute toxicity and biochemical pharmacological effects of Agkistrodon acutus venom thrombin-like enzyme on dogs and rabbits were reported. Dosage (μg / kg · d) were 200,65 dogs, respectively, 540,180 rabbits, continuous intravenous injection of 28 days. At 14 and 28 days after treatment, WBC, DC, Hb content, liver and arm function were not significantly different. Plasma fibrinogen content was significantly reduced, thrombin-like enzyme time and thrombin time was significantly prolonged, platelet aggregation was significantly inhibited, high-dose group of individual dog blood urea nitrogen increased significantly (reduced after 2 days of withdrawal), the end of the experiment were increased body weight . Ten days after treatment, individual dogs showed a short-term reaction after daily injection, and disappeared after 3 weeks. Pathological anatomy of the eye meat and microscopic examination did not occur in important organs disease. The data suggest that thrombin-like enzyme is a low toxicity and high efficacy of agents, inhibition of platelet aggregation after 2 weeks of treatment with tolerance, so continuous treatment time should not be too long.