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目的观察经腹腔注射的新城疫病毒(NDV)对小鼠脾脏NK细胞表达肿瘤坏死因子相关凋亡诱导配体(TRAIL)及杀伤Novikoff小鼠肝癌细胞的影响,并探讨其与γ干扰素(IFN-γ)的关系。方法给予BALB/c小鼠和IFN-γR-/-B6.129S7小鼠腹腔注射NDV,12 h后,用ELISA检测小鼠外周血IFN-γ浓度;分离小鼠脾脏NK细胞,用反转录PCR法检测NK细胞中TRAIL mRNA转录水平,Western blot法检测脾脏NK细胞中TRAIL蛋白水平,用乳酸脱氢酶(LDH)释放法检测NK细胞对Novikoff肝癌细胞的杀伤作用。结果腹腔注射NDV可以提高BALB/c小鼠外周血IFN-γ浓度,上调小鼠脾脏NK细胞TRAIL mRNA和蛋白表达水平,增强小鼠脾脏NK细胞体外条件下对Novikoff肝癌细胞的杀伤活性。TRAIL中和抗体能抑制NK细胞对Novikoff肝癌细胞的杀伤作用。IFN-γR-/-B6.129S7小鼠接受NDV注射后脾脏NK细胞的TRAIL表达无显著增加,NDV激活的NK细胞对Novikoff肝癌细胞的杀伤活性显著低于IFN-γ受体正常的BALB/c小鼠。结论腹腔注射NDV可增强脾脏NK细胞的体外抗肿瘤活性,其机制之一是通过IFN-γ受体途径上调NK细胞表面TRAIL蛋白表达来发挥作用。
Objective To investigate the effect of NDV on the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and the killing of Novikoff mouse hepatoma cells in mice spleen NK cells. -γ). Methods BALB / c mice and IFN-γR - / - B6.129S7 mice were intraperitoneally injected with NDV. After 12 hours, the concentrations of IFN-γ in peripheral blood of mice were detected by ELISA. The spleen NK cells were isolated and transfected by reverse transcription The transcription level of TRAIL mRNA in NK cells was detected by PCR, the level of TRAIL protein in spleen NK cells was detected by Western blot, and the killing effect of NK cells on Novikoff hepatoma cells was detected by lactate dehydrogenase (LDH) release assay. Results Intraperitoneal injection of NDV increased the concentration of IFN-γ in peripheral blood of BALB / c mice and up-regulated the expression of TRAIL mRNA and protein in splenic NK cells of mice and enhanced the killing activity of NK cells on Novikoff hepatoma cells in vitro. TRAIL neutralizing antibodies can inhibit NK cell killing of Novikoff liver cancer cells. No significant increase of TRAIL expression was observed in splenic NK cells after NDV injection in IFN-γR - / - B6.129S7 mice, while the killing activity of NDV-activated NK cells on Novikoff hepatoma cells was significantly lower than that of normal BALB / c mice Mouse. Conclusion Intraperitoneal injection of NDV can enhance the anti-tumor activity of splenic NK cells in vitro. One of the mechanisms is that IFN-γ receptor pathway upregulates TRAIL expression on NK cells.